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Screen new cultures as they arrive, and existing stocks at regular intervals, eg, once a month There are a number of tests available, but the most reliable and sensitive are uorescence microscopy after staining with Hoechst 33258 [Chen et al, 1977; Protocol 192, Freshney, 2005] or PCR with a panmycoplasma primer [Uphoff and Drexler, 2002a; Protocol 193, Freshney, 2005] The latter is more sensitive but depends on the availability of PCR technology, whereas the former is easier, will detect any DNA-containing contamination, but requires a uorescence microscope Both techniques are best performed with so-called indicator cultures The test culture is refreshed with antibiotic-free medium and, after 3 5 days, the medium is transferred to an antibiotic-free, 10% con uent indicator culture of a cell line such as 3T6 or A549 cells, which are well spread and known to support mycoplasma growth.

Membership rose steadily from the founding of the NASP and stood at 21,000 plus in 2000 The association developed an impressive track record of working to raise educational and training standards Accreditation of training programs became an important part of the NASP s work as well (Fagan, Gorin, & Tharinger, 2000) Applied psychological organizations proliferated in the last decades of the twentieth century Space does not allow for all of them to be described A few chosen to serve as examples include the American Association of Correctional Psychologists (founded 1953), the International Society of Sport Psychology (founded 1965), the Association for the Advancement of Behavior Therapy (founded 1966), the Association for Applied Psychophysiology and Biofeedback (founded 1969), and the Society of Psychologists in Management (founded 1984) Hundreds of other applied psychological organizations had been established in the United States alone by the end of the twentieth century (see VandenBos, 1989).

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After a further 3 days (the indicator cells must not be allowed to reach con uence) the indicator culture is xed in acetic methanol and stained with Hoechst 33258, or harvested by scraping for PCR (trypsinization may remove the mycoplasma from the cell surface) Discard all contaminated cultures If the culture is irreplaceable, decontamination may be attempted (under strict quarantine conditions) with agents.

Psychological Organizations in the Public Interest The 1960s were a time of social upheaval and dissent in the United States and much of Europe During this period, psychological organizations were formed to represent groups that had been traditionally ignored or underrepresented in mainstream psychological organizations In the United States, these new organizations were typically formed as a response to perceived problems with the APA The APA was perceived as insensitive to the needs and interests of women psychologists and psychologists of color The Association of Black Psychologists (ABPsi) was founded in 1968 at the annual convention of the APA It was not the rst organization of African American psychologists In.

where rY1 and rY2 are the correlations of Predictors 1 and 2 with the criterion, respectively, and r12 is the correlation between the predictors. The expression for bY 1.2 includes both the correlation of predictor in question (rY1) and the correlation of the

tone or set of tones, or simply the beginning of a voice prompt announcing that the caller is now in the identi cation phase. Here again, the identi cation of voice begin may be easier than pinning down the tone or set of tones, especially when IP transport is used. This sequence is shown in Figure A-5. If the identi cation is successful, the caller can proceed to the next stage, and an IVR or dial tone will be heard. A failed identi cation may result in a busy tone or a di erent IVR message. It is then necessary to determine the response time needed to allow the user to provide input for authentication (e.g., via the use of a 4- to 8-digit PIN). The response could be a standard dial tone, a vendor-speci c tone or set of tones, or simply the beginning of a voice prompt announcing the result of authentication. Here again, the identi cation of voice begin may be easier than pinning down the tone or set of tones, especially when IP transport is used. The steps are shown in Figure A-6. If the authentication is successful, the caller can proceed to the next stage, and an IVR or dial tone will be heard. A failed authentication may result in a busy or fast-busy tone or a voice announcement.

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